Automated ELISA for direct measurement of free 25OH vitamin D

Multicenter Comparison of Seven 25OH Vitamin D Automated Immunoassays

BACKGROUND The measurement of 25OH vitamin D continues to grow in clinical laboratories. The aim of this multi-center study was to compare the results of seven automated commercial immunoassays with a reference HPLC technique. METHODS One hundred and twenty consecutive outpatient serum samples were centrifuged, divided in aliquots, frozen and shipped to the participating laboratories. 25OH Vi...

Sensitive automated ELISA for measurement of vitamin D-binding protein (Gc) in human urine.

an Italian multicentre study. Italian Society of Paediatric Gastroenterology and Hepatology (SIGEP) and “Club del Tenue” Working Groups on Coeliac Disease. Gut 1998;42:362–5. 4. Collin P, Maki M, Keyrilainen O, Hallstrom O, Reunala T, Pasternack A. Selective IgA deficiency and coeliac disease. Scand J Gastroenterol 1992; 27:367–71. 5. Hill PG, Forsyth JM, Semeraro D, Holmes GK. IgA antibodies t...

Effects of Antiepileptic Drug Therapy on 25oh Vitamin D Levels

Background: Epilepsy is a chronic condition with an incidence of 4–10/1,000 worldwide, Antiepileptic drugs (AED) in common use are carbamazepine (CBZ), phenytoin (DPH), phenobarbitone (PB), and sodium valproate. The effect of AEDs on the vitamin D metabolism is of considerable value especially in children. There are few clinicians who use prophylactic calcium or vitamin D for patients taking AE...

An automated microbiological method for the measurement of vitamin B12.

An automated method for the microbiological assay of vitamin B(12) is described. A chloramphenicol-resistant strain of Lactobacillus leichmannii is used as the test organism and this eliminates the need for sterilization or aseptic addition. Precipitation of the serum protein is avoided by dilution of the serum with a glutamic/malic acid solution before heating the preparation to free the vitam...

Vitamin D: clinical measurement and use.